Rapid Detection of Uropathogens and Antibiotic-resistant Genes Using Open Array Multiplex PCR Technology

Abstract Introduction/Objective Introduction: Standard diagnostic practices for Urinary Tract Infection (UTI) take 2–3 days pathogen identification and antibiotic susceptibility testing leading to delayed diagnosis an increase in healthcare expenditures. The rapid diagnostics tool is highly desirable identify the its antibiotic-resistant genes directly from samples. Our objective validate evaluate performance of Open array multiplex PCR resistance genes. Methods/Case Report Method: method 20 UTI pathogens was validated using ATCC organisms inter-laboratory correlation 50 urine Briefly, samples were homogenized Omni bead ruptor 96, DNA extracted Hamilton Vantage. Customized open chips with most common urinary tract target 13 resistant specifically treatment filled Taqman master mix a 1:1 ratio. prepared on Accufill real-time performed Quantstudio 12K flex. A total 116 de-identified patient analyzed. Results (if Case Study enter NA) Result: sensitivity, specificity, accuracy this more than 90% considering reference as standard method. 14 different types detected. Escherichia coli (n=49) highest among all. Multiple identified 27 Organisms such Ureaplasma Megasphaera 1 detected nine four samples, respectively. All positive at least one or two tetM tetS 72 erm A, B, C 60 ESBL 30 fosfo 21 Conclusion Conclusion: technology may help early that can improve care, shorten hospitalization, reduces economic burden.